1、首先準(zhǔn)備好受血者和獻(xiàn)血者的血樣。

2、配好用受血者血清配成的2%的紅細(xì)胞懸液；用獻(xiàn)血者血清配成的2%紅細(xì)胞懸液。

3、主側(cè)加受血者血清0.5ml，獻(xiàn)血者血清配成的2%紅細(xì)胞懸液0.25ml.

4、次側(cè)加獻(xiàn)血者血清0.5ml，受血者血清配成的2%的紅細(xì)胞懸液0.25ml.

5、37度水浴箱孵育1小時(shí)，后觀察有無(wú)凝集或溶血。

如有配血禁忌，則應(yīng)查原因。